![]() For further enzymatic reactions remove SDS or EDTA by spin column-based PCR purification kit. Proteins in this family are typically between 270 and 341 amino acids in length. ![]() enzymes with CpG in their recognition site, such as EagI, NotI, and SalI. ![]() Each restriction enzyme recognizes specific DNA sequences, and cleavage can occur within the recognition sequence or some distance away, depending on the enzyme. Restriction endonucleases are bacterial enzymes that bind and cleave DNA at. Alternatively use our environmentally friendly 6x orange gel loading buffer. In general, restriction enzymes cleave double-stranded DNA. Restriction enzymes enable a DNA molecule to be cut at a specific location and. NotI is a restriction enzyme purified from Nocardia otitidis-caviarum that cleaves the palindromic sequence GCGGCCGC. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).Contents 10x U NotI: 500 mM Tris-HCl (pH 7.9 at 25 ☌), 1000mM NaCl, 50mM MgCl2, 1mg/mL BSAġ0mM or SDS to a concentration of 0.1 %. Restriction enzymes (NotI, SfiI) were purchased from USB (Cleveland, Ohio, USA). Choose your restriction enzyme by overhang type, recognition site size. Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. It is also used to quickly check the identity of a plasmid by diagnostic digest. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. The enzyme cuts the double-stranded DNA, resulting in DNA fragments. Each restriction enzyme moves along a DNA molecule until it finds a specific recognition sequence in the DNA. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Restriction enzyme digestion is commonly used in molecular cloning techniques, such as PCR or restriction cloning. Restriction enzymes (also called restriction endonucleases) are proteins made by many bacterial species, to defend against viral infections. The enzyme is also fully active in the respective buffers from other suppliers. Concentration: 10U/µL Source: Nocardia corallina Reagents supplied with: The Enzyme is supplied with 10x Unique NotI Buffer. ), provide a unique insight into the structural details of 8 base pair sequence recognition by the restriction enzyme. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. NotI is a restriction enzyme purified from Nocardia otitidis-caviarum that cleaves the palindromic sequence GC GGCCGC.
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